Aurora Kinase B antibody (pThr232)

Catalog No. ABIN1531642

This Rabbit Polyclonal antibody specifically detects Aurora Kinase B in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Aurora Kinase B antibody (pThr232) (ABIN1531642)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 198-247, pThr232

Specificity:

AurB (Phospho-Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Thr232.

PhosphorylationH:T232 M:T237 R:T235

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AurB around the phosphorylation site of Thr232.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.442658 (NCBI Gene Symbol: AURKB)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AurB

Background:

Synonyms: AIK2, AIM-1, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Serine/threonine protein kinase 12, STK-1, STK12

NCBI Gene Symbol: AURKB

Molecular Weight: 39 kDa

Gene ID: 9212

OMIM: 604970

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades