IL-10RA antibody (pTyr496)

Catalog No. ABIN1531662

The Rabbit Polyclonal anti-IL-10RA antibody (ABIN1531662) specifically detects IL-10RA in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IL-10RA antibody (pTyr496) (ABIN1531662)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10RA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-10RA Antibody

Binding Specificity: AA 462-511, pTyr496

Purpose: IL-10Rα (phospho Tyr496) Antibody

Specificity: Phospho-IL-10Rα (Y496) Polyclonal Antibody detects endogenous levels of IL-10Rα protein only when phosphorylated at Y496.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IL-10R alpha around the phosphorylation site of Tyr496. AA range:462-511

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: IL-10R alpha

Background:

Synonyms: IL10RA, IL10R, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, CD antigen CD210

Background: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009],

Gene Name: IL10RA

Protein Name: Interleukin-10 receptor subunit alpha

Molecular Weight: 62 kDa

Gene ID: 3587

OMIM: 146933

UniProt: Q13651

Pathways: Growth Factor Binding