IL7R antibody (pTyr449)

Catalog No. ABIN1531668

Product Details anti-IL7R Antibody

Target: IL7R (Interleukin 7 Receptor (IL7R))

Binding Specificity: AA 410-459, pTyr449

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity:

IL-7R/CD127 (Phospho-Tyr449) Antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at Tyr449.

PhosphorylationH:Y449 M:Y449

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.591742, Hs.635723 (NCBI Gene Symbol: IL7RA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL-7R/CD127 (IL7R Products)

Background:

Synonyms: CD127 antigen, CDw127, IL-7R alpha, IL7RA, interleukin-7 receptor alpha chain

NCBI Gene Symbol: IL7RA

Molecular Weight: 51 kDa

Gene ID: 3575

OMIM: 126200

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size