Aconitase 1 antibody (pSer711)

Catalog No. ABIN1531674

The Rabbit Polyclonal anti-Aconitase 1 antibody (ABIN1531674) specifically detects Aconitase 1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Aconitase 1 antibody (pSer711) (ABIN1531674)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: AA 681-730, pSer711

Purpose: IRP-1 (phospho Ser711) Antibody

Specificity: Phospho-IRP-1 (S711) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S711.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711. AA range:681-730

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: IREB1

Background:

Synonyms: ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct

Gene Name: ACO1

Protein Name: Cytoplasmic aconitate hydratase

Molecular Weight: 98 kDa

Gene ID: 48

OMIM: 100880

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis