Topoisomerase II alpha antibody (pSer1106)

Catalog No. ABIN1531703

Product Details anti-Topoisomerase II alpha Antibody

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Binding Specificity: AA 1081-1130, pSer1106

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity:

TOP2A (Phospho-Ser1106) Antibody detects endogenous levels of TOP2A only when phosphorylated at Ser1106.

PhosphorylationH:S1106

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Ser1106.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.156346 (NCBI Gene Symbol: TOP2A)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A (TOP2A Products)

Background:

Synonyms: alpha isozyme, DNA topoisomerase II, DNA topoisomerase II-alpha, P11388-1, TOP-2, TOP2, TOP2A, TP2A

NCBI Gene Symbol: TOP2A

Molecular Weight: 174 kDa

Gene ID: 7153

OMIM: 126430

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases