Proteasome alpha3 (AA 206-255), (pSer250) antibody

Catalog No. ABIN1531751

The Rabbit Polyclonal anti-Proteasome alpha3 antibody is suitable to detect Proteasome alpha3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Proteasome alpha3 (AA 206-255), (pSer250) antibody (ABIN1531751)

Target: Proteasome alpha3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 206-255, pSer250

Purpose: 20S Proteasome α3 (phospho Ser250) Antibody

Specificity: Phospho-20S Proteasome α3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome α3 protein only when phosphorylated at S250.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: Proteasome alpha3

Background:

Synonyms: PSMA3, HC8, PSC8, Proteasome subunit alpha type-3, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

Gene Name: PSMA3

Protein Name: Proteasome subunit alpha type-3

Molecular Weight: 28 kDa

Gene ID: 5684

OMIM: 176843

UniProt: P25788