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Vimentin antibody (pSer56)

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, IHC and ELISA. It is suitable to detect Vimentin in samples from Human.
Catalog No. ABIN1531760

Quick Overview for Vimentin antibody (pSer56) (ABIN1531760)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Binding Specificity

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    AA 31-80, pSer56

    Specificity

    Vimentin (Phospho-Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.

    PhosphorylationH:S56

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

    Comment

    Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Synonyms: VIME, vimentin

    NCBI Gene Symbol: VIM

    Molecular Weight

    53 kDa

    Gene ID

    7431

    OMIM

    193060

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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