BCR antibody (pTyr177)

Catalog No. ABIN1531789

This Rabbit Polyclonal antibody specifically detects BCR in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for BCR antibody (pTyr177) (ABIN1531789)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BCR Antibody

Binding Specificity: AA 144-193, pTyr177

Purpose: Bcr (phospho Tyr177) Antibody

Specificity: Phospho-Bcr (Y177) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y177.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr177. AA range:144-193

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: Bcr

Background:

Synonyms: BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

Background: A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: BCR

Protein Name: Breakpoint cluster region protein

Molecular Weight: 142 kDa

Gene ID: 613

OMIM: 151410

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling