DOK2 antibody (pTyr299)

Catalog No. ABIN1531817

The Rabbit Polyclonal anti-DOK2 antibody is suitable to detect DOK2 in samples from Human, Mouse and Monkey. It has been validated for WB, ELISA and IF.

Quick Overview for DOK2 antibody (pTyr299) (ABIN1531817)

Target: DOK2

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-DOK2 Antibody

Binding Specificity: AA 266-315, pTyr299

Purpose: Dok-2 (phospho Tyr299) Antibody

Specificity: Phospho-Dok-2 (Y299) Polyclonal Antibody detects endogenous levels of Dok-2 protein only when phosphorylated at Y299.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p56 Dok-2 around the phosphorylation site of Tyr299. AA range:266-315

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DOK2

Target: DOK2

Alternative Name: p56 Dok-2

Background:

Synonyms: DOK2, Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2)

Background: docking protein 2(DOK2) Homo sapiens The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008],

Gene Name: DOK2

Protein Name: Docking protein 2

Molecular Weight: 45 kDa

Gene ID: 9046

OMIM: 604997

UniProt: O60496