ERK1/2 antibody (pThr202)

Catalog No. ABIN1531831

This Rabbit Polyclonal antibody specifically detects ERK1/2 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERK1/2 antibody (pThr202) (ABIN1531831)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-ERK1/2 Antibody

Binding Specificity: AA 169-218, pThr202

Purpose: ERK 1/2 (phospho Thr202) Antibody

Specificity: Phospho-ERK 1/2 (T202) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Thr202. AA range:169-218

Isotype: IgG

Application Details

Application Notes: IF 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: p44/42 MAP Kinase

Background:

Synonyms: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p

Background: The protein encoded byThis gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

Gene Name: MAPK1/MAPK3

Protein Name: Mitogen-activated protein kinase 3

Molecular Weight: 43 kDa

Gene ID: 5595, 5594

OMIM: 601795

UniProt: P27361