Ezrin antibody (pThr566)

Catalog No. ABIN1531837

This Rabbit Polyclonal antibody specifically detects Ezrin in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ezrin antibody (pThr566) (ABIN1531837)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ezrin Antibody

Binding Specificity: AA 534-583, pThr566

Purpose: Ezrin (phospho Thr566) Antibody

Specificity: Phospho-Ezrin (T566) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at T566.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Thr566. AA range:534-583

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background:

Synonyms: EZR, VIL2, Ezrin, Cytovillin, Villin-2, p81

Background: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008],

Gene Name: EZR

Protein Name: Ezrin

Molecular Weight: 69 kDa

Gene ID: 7430

OMIM: 123900

UniProt: P15311

Pathways: Maintenance of Protein Location