Ezrin antibody (pTyr353)

Catalog No. ABIN1531838

The Rabbit Polyclonal anti-Ezrin antibody is suitable to detect Ezrin in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Ezrin antibody (pTyr353) (ABIN1531838)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ezrin Antibody

Binding Specificity: AA 321-370, pTyr353

Purpose: Ezrin (phospho Tyr353) Antibody

Specificity: Phospho-Ezrin (Y353) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y353.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Tyr353. AA range:321-370

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background:

Synonyms: EZR, VIL2, Ezrin, Cytovillin, Villin-2, p81

Background: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008],

Gene Name: EZR

Protein Name: Ezrin

Molecular Weight: 69 kDa

Gene ID: 7430

OMIM: 123900

UniProt: P15311

Pathways: Maintenance of Protein Location