ErbB2/Her2 antibody (pTyr877)

Catalog No. ABIN1531861

This Rabbit Polyclonal antibody specifically detects ErbB2/Her2 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ErbB2/Her2 antibody (pTyr877) (ABIN1531861)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: AA 851-900, pTyr877

Specificity:

HER2 (Phospho-Tyr877) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyr877.

PhosphorylationH:Y877 M:Y878 R:Y879

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr877.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.446352 (NCBI Gene Symbol: ERBB2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: HER2

Background:

Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, kinase ErbB2, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell s

NCBI Gene Symbol: ERBB2

Molecular Weight: 137 kDa

Gene ID: 2064

OMIM: 164870

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development