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HSF1 antibody (pSer303)

The Rabbit Polyclonal anti-HSF1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect HSF1 in samples from Human and Mouse.
Catalog No. ABIN1531865

Quick Overview for HSF1 antibody (pSer303) (ABIN1531865)

Target

See all HSF1 Antibodies
HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity

  • 145
  • 89
  • 40
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  • 4
  • 4
  • 1
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Human, Mouse

Host

  • 134
  • 38
  • 8
  • 1
Rabbit

Clonality

  • 120
  • 60
Polyclonal

Conjugate

  • 107
  • 10
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  • 5
  • 5
  • 2
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This HSF1 antibody is un-conjugated

Application

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  • 10
  • 5
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Binding Specificity

    • 16
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    AA 270-319, pSer303

    Specificity

    HSF1 (Phospho-Ser303) Antibody detects endogenous levels of HSF1 only when phosphorylated at Ser303.

    PhosphorylationH:S303 M:S303

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

    Comment

    Unigene-Number: Hs.530227, Hs.571397 (NCBI Gene Symbol: HSF1)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    HSF1 (Heat Shock Factor Protein 1 (HSF1))

    Alternative Name

    HSF1

    Background

    Synonyms: Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSTF 1, HSTF1

    NCBI Gene Symbol: HSF1

    Molecular Weight

    57 kDa

    Gene ID

    3297

    OMIM

    140580

    UniProt

    Q00613
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