HSP27 antibody (pSer15)

Catalog No. ABIN1531866

This anti-HSP27 antibody is a Rabbit Polyclonal antibody detecting HSP27 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for HSP27 antibody (pSer15) (ABIN1531866)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HSP27 Antibody

Binding Specificity: AA 5-54, pSer15

Specificity:

HSP27 (Phospho-Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser15.

PhosphorylationH:S15

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.520973 (NCBI Gene Symbol: HSPB1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background:

Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27

NCBI Gene Symbol: HSPB1

Molecular Weight: 22 kDa

Gene ID: 3315

OMIM: 602195

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling