IRS1 antibody (pSer307)

Catalog No. ABIN1531876

This Rabbit Polyclonal antibody specifically detects IRS1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for IRS1 antibody (pSer307) (ABIN1531876)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-IRS1 Antibody

Binding Specificity: AA 274-323, pSer307

Specificity:

IRS-1 (Phospho-Ser307) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser307.

PhosphorylationH:S307 M:S302 R:S302

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser307.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.471508 (NCBI Gene Symbol: IRS1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-IRS1 antibody (ABIN1531876)

Simon-Szabó, Kokas, Mandl, Kéri, Csala: "Metformin attenuates palmitate-induced endoplasmic reticulum stress, serine phosphorylation of IRS-1 and apoptosis in rat insulinoma cells." in: PLoS ONE, Vol. 9, Issue 6, pp. e97868, (2014) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS-1

Background:

Synonyms: Insulin receptor substrate-1, IRS-1, IRS1

NCBI Gene Symbol: IRS1

Molecular Weight: 131 kDa

Gene ID: 3667

OMIM: 125853

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process