PDPK1 antibody (AA 210-259)

Catalog No. ABIN1531949

The Rabbit Polyclonal anti-PDPK1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect PDPK1 in samples from Human, Mouse and Rat.

Quick Overview for PDPK1 antibody (AA 210-259) (ABIN1531949)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 210-259

Specificity:

PDK1 (Phospho-Ser241) Antibody detects endogenous levels of PDK1 only when phosphorylated at Ser241.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Ser241.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.459692 (NCBI Gene Symbol: PDPK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background:

Synonyms: 3-phosphoinositide dependent protein kinase-1, hPDK1, kinase PDK1, mPDK1, PDPK1, PkB kinase, Protein kinase B kinase

NCBI Gene Symbol: PDPK1

Molecular Weight: 63 kDa

Gene ID: 5170

OMIM: 605213

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals