Src antibody (pTyr530)

Catalog No. ABIN1531972

This Rabbit Polyclonal antibody specifically detects Src in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Src antibody (pTyr530) (ABIN1531972)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Src Antibody

Binding Specificity: AA 487-536, pTyr530

Specificity:

Src (Phospho-Tyr529) Antibody detects endogenous levels of Src only when phosphorylated at Tyr529.

PhosphorylationH:Y530 M:Y535 R:Y536

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr529.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.195659 (NCBI Gene Symbol: SRC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Background:

Synonyms: c-Src, C-SRC, kinase Src, Neuronal SRC, p60-SRC, p60-Src, Src, SRC, SRC1

NCBI Gene Symbol: SRC

Molecular Weight: 59 kDa

Gene ID: 6714

OMIM: 190090

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling