VAV1 antibody (pTyr174)

Catalog No. ABIN1531997

The Rabbit Polyclonal anti-VAV1 antibody has been validated for WB and ELISA. It is suitable to detect VAV1 in samples from Human, Rat and Mouse.

Quick Overview for VAV1 antibody (pTyr174) (ABIN1531997)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VAV1 Antibody

Binding Specificity: AA 141-190, pTyr174

Specificity:

VAV1 (Phospho-Tyr174) Antibody detects endogenous levels of VAV1 only when phosphorylated at Tyr174.

PhosphorylationH:Y174 M:Y174 R:Y174

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human VAV1 around the phosphorylation site of Tyr174.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.116237 (NCBI Gene Symbol: VAV1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Alternative Name: VAV1

Background:

Synonyms: Proto-oncogene vav, VAV1, VAV

NCBI Gene Symbol: VAV1

Molecular Weight: 98 kDa

Gene ID: 7409

OMIM: 164875

UniProt: P15498

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling