HSF1 antibody (pThr142)

Catalog No. ABIN1532015

The Rabbit Polyclonal anti-HSF1 antibody has been validated for ELISA and IHC. It is suitable to detect HSF1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for HSF1 antibody (pThr142) (ABIN1532015)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-HSF1 Antibody

Binding Specificity: AA 108-157, pThr142

Specificity:

HSF1 (Phospho-Thr142) Antibody detects endogenous levels of HSF1 only when phosphorylated at Thr142.

PhosphorylationH:T142 M:T142

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.530227, Hs.571397 (NCBI Gene Symbol: HSF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-HSF1 antibody (ABIN1532015)

Tamaru, Hattori, Ninomiya, Kawamura, Varès, Honda, Mishra, Wang, Benjamin, Sassone-Corsi, Ozawa, Takamatsu: "ROS Stress Resets Circadian Clocks to Coordinate Pro-Survival Signals." in: PLoS ONE, Vol. 8, Issue 12, pp. e82006, (2013) (PubMed).

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Synonyms: Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSTF 1, HSTF1

NCBI Gene Symbol: HSF1

Molecular Weight: 57 kDa

Gene ID: 3297

OMIM: 140580

UniProt: Q00613