LIMK2 antibody (pSer283)

Catalog No. ABIN1532018

The Rabbit Polyclonal anti-LIMK2 antibody is suitable to detect LIMK2 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for LIMK2 antibody (pSer283) (ABIN1532018)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-LIMK2 Antibody

Binding Specificity: AA 249-298, pSer283

Purpose: LIMK-2 (phospho Ser283) Antibody

Specificity: Phospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human LIMK2 around the phosphorylation site of Ser283. AA range:249-298

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background:

Synonyms: LIMK2, LIM domain kinase 2, LIMK-2

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: LIMK2

Protein Name: LIM domain kinase 2

Molecular Weight: 72 kDa

Gene ID: 3985

OMIM: 601988

UniProt: P53671