AKT1/3 (AA 406-455), (pTyr437) antibody

Catalog No. ABIN1532035

Product Details

Target: AKT1/3

Binding Specificity: AA 406-455, pTyr437

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

AKT1/3 (Phospho-Tyr437/434) Antibody detects endogenous levels of AKT1/3 only when phosphorylated at Tyr437/434.

PhosphorylationH:Y437/434 M:Y437/434 R:Y437/434

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AKT1/3 around the phosphorylation site of Tyr437/434.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.525622, Hs.631535, Hs.498292 (NCBI Gene Symbol: AKT1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details

Target: AKT1/3

Background:

Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT

NCBI Gene Symbol: AKT1

Molecular Weight: 55 kDa

Gene ID: 207, 208, 10000

OMIM: 164730

UniProt: P31749, Q9Y243