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MLK1/2 antibody (pThr312)

The Rabbit Polyclonal anti-MLK1/2 antibody has been validated for IHC and ELISA. It is suitable to detect MLK1/2 in samples from Human and Mouse.
Catalog No. ABIN1532062

Quick Overview for MLK1/2 antibody (pThr312) (ABIN1532062)

Target

MLK1/2

Reactivity

Human, Mouse

Host

  • 12
Rabbit

Clonality

  • 12
Polyclonal

Conjugate

  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MLK1/2 antibody is un-conjugated

Application

  • 5
  • 3
  • 3
Immunohistochemistry (IHC), ELISA
  • Binding Specificity

    • 10
    • 9
    • 2
    • 1
    • 1
    AA 281-330, pThr312

    Specificity

    MLK1/2 (Phospho-Thr312/266) Antibody detects endogenous levels of MLK1/2 only when phosphorylated at Thr312/266.

    PhosphorylationH:T312/266 M:T305/266

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266.

    Isotype

    IgG
  • Application Notes

    IHC: 1:50~1:100 ELISA: 1:5000

    Comment

    Unigene-Number: Hs.445496, Hs.593542, Hs.466743 (NCBI Gene Symbol: MAP3K9)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    MLK1/2

    Background

    Synonyms: Mixed lineage kinase 1/ Mixed lineage kinase 2, Protein kinase MST

    NCBI Gene Symbol: MAP3K9

    Molecular Weight

    121 kDa

    Gene ID

    4293, 4294

    OMIM

    600136

    UniProt

    P80192, Q02779
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