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RSK1/2/3/4 (AA 191-240), (pSer221) antibody

This Rabbit Polyclonal antibody specifically detects in WB and ELISA. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN1532079

Quick Overview for RSK1/2/3/4 (AA 191-240), (pSer221) antibody (ABIN1532079)

Target

RSK1/2/3/4

Reactivity

Human, Mouse

Host

  • 13
Rabbit

Clonality

  • 13
Polyclonal

Conjugate

  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Un-conjugated

Application

Western Blotting (WB), ELISA
  • Binding Specificity

    • 10
    • 9
    • 9
    • 9
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 191-240, pSer221

    Specificity

    RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.

    PhosphorylationH:S221/227/S218/232 M:S221/227/S218/252

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 ELISA: 1:40000

    Comment

    Unigene-Number: Hs.149957, Hs.445387, Hs.655277, Hs.368153 (NCBI Gene Symbol: RPS6KA1)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    RSK1/2/3/4

    Background

    Synonyms: S6K-alpha 1, 90 kDa ribosomal protein S6 kinase 1, p90-RSK 1, Ribosomal S6 kinase 1, RSK-1, pp90RSK1, p90S6K, MAP kinase-activated protein kinase 1a, MAPKAPK1A/ S6K-alpha 3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, Ribosomal S6 kinase 2, RSK-2, pp

    NCBI Gene Symbol: RPS6KA1

    Molecular Weight

    82 kDa

    Gene ID

    6195, 6197, 6196

    OMIM

    601684

    UniProt

    Q15418, P51812, Q15349, Q9UK32
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