RSK1/2/3/4 (pSer221), (pSer227) antibody

Catalog No. ABIN7224092

This Rabbit Polyclonal antibody specifically detects RSK1/2/3/4 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RSK1/2/3/4 (pSer221), (pSer227) antibody (ABIN7224092)

Target: RSK1/2/3/4

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: pSer221, pSer227

Purpose: Rsk-1/2/3/4 (phospho Ser221/227/S218/232) Polyclonal Antibody

Specificity: Phospho-Rsk-1/2/3/4 (S221/227/S218/232) Polyclonal Antibody detects endogenous levels of Rsk-1/2/3/4 protein only when phosphorylated at S221/227/S218/232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rsk-1/2/3/4 Phospho-Ser221/227/S218/232

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: RSK1/2/3/4

Alternative Name: Rsk-1/2/3/4

Background: Rabbit Anti-Rsk-1/2/3/4 (phospho Ser221/227/S218/232) Polyclonal Antibody,RPS6KA1, MAPKAPK1A, RSK1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAP,RPS6KA1 (ribosomal protein S6 kinase A1) encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,Ribosomal protein S6 kinase alpha-1

Molecular Weight: observerd band 85kDa

Gene ID: 6195, 6197, 6196