NOS2 antibody (pTyr151)

Catalog No. ABIN1532100

This Rabbit Polyclonal antibody specifically detects NOS2 in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NOS2 antibody (pTyr151) (ABIN1532100)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NOS2 Antibody

Binding Specificity: AA 117-166, pTyr151

Purpose: iNOS (phospho Tyr151) Antibody

Specificity: Phospho-NOS2 (Y151) Polyclonal Antibody detects endogenous levels of NOS2 protein only when phosphorylated at Y151.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS around the phosphorylation site of Tyr151. AA range:117-166

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: iNOS

Background:

Synonyms: NOS2, NOS2A, Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2

Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities.This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

Gene Name: NOS2, INOS

Protein Name: Nitric oxide synthase inducible

Molecular Weight: 131 kDa

Gene ID: 4843

OMIM: 163730

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process