Src antibody (pTyr216)

Catalog No. ABIN1532102

This anti-Src antibody is a Rabbit Polyclonal antibody detecting Src in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Src antibody (pTyr216) (ABIN1532102)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Src Antibody

Binding Specificity: AA 181-230, pTyr216

Specificity:

Src (Phospho-Tyr216) Antibody detects endogenous levels of Src only when phosphorylated at Tyr216.

PhosphorylationH:Y216 M:Y221 R:Y216

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr216.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.195659 (NCBI Gene Symbol: SRC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Background:

Synonyms: c-Src, C-SRC, kinase Src, Neuronal SRC, p60-SRC, p60-Src, Src, SRC, SRC1

NCBI Gene Symbol: SRC

Molecular Weight: 59 kDa

Gene ID: 6714

OMIM: 190090

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling