RUNX1 antibody (pSer435)

Catalog No. ABIN1532121

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RUNX1 antibody (pSer435) (ABIN1532121)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX1 Antibody

Binding Specificity: AA 401-450, pSer435

Specificity:

AML1 (Phospho-Ser435) Antibody detects endogenous levels of AML1 only when phosphorylated at Ser435.

PhosphorylationH:S435 M:S435 R:S434

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AML1 around the phosphorylation site of Ser435.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.149261, Hs.612648 (NCBI Gene Symbol: RUNX1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1

Background:

Synonyms: Acute myeloid leukemia 1 protein, CBF-alpha 2, CBFA2, Core-binding factor, alpha 2 subunit, Oncogene AML-1, PEA2-alpha B, PEBP2-alpha B, Polyomavirus enhancer binding protein 2 alpha B subunit, RUN1, Runt-related transcription factor 1, RUNX1, SL3-3 enhan

NCBI Gene Symbol: RUNX1

Molecular Weight: 48 kDa

Gene ID: 861

OMIM: 151385

UniProt: Q01196