Claudin 3 antibody (pTyr219)

Catalog No. ABIN1532131

The Rabbit Polyclonal anti-Claudin 3 antibody is suitable to detect Claudin 3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Claudin 3 antibody (pTyr219) (ABIN1532131)

Target: Claudin 3 (CLDN3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Claudin 3 Antibody

Binding Specificity: AA 171-220, pTyr219

Purpose: Claudin-3 (phospho Tyr219) Antibody

Specificity: Phospho-Claudin-3 (Y219) Polyclonal Antibody detects endogenous levels of Claudin-3 protein only when phosphorylated at Y219.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Claudin 3 around the phosphorylation site of Tyr219. AA range:171-220

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Claudin 3

Target: Claudin 3 (CLDN3)

Alternative Name: Claudin 3

Background:

Synonyms: CLDN3, C7orf1, CPETR2, Claudin-3, Clostridium perfringens enterotoxin receptor 2, CPE-R 2, CPE-receptor 2, Rat ventral prostate.1 protein homolog, hRVP1

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008],

Gene Name: CLDN3

Protein Name: Claudin-3

Molecular Weight: 23 kDa

Gene ID: 1365

OMIM: 602910

UniProt: O15551

Pathways: Hepatitis C