Doublecortin antibody (pSer376)

Catalog No. ABIN1532140

The Rabbit Polyclonal anti-Doublecortin antibody is suitable to detect Doublecortin in samples from Human, Mouse, Rat and Pig. It has been validated for ELISA, IHC and IF.

Quick Overview for Doublecortin antibody (pSer376) (ABIN1532140)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Doublecortin Antibody

Binding Specificity: AA 330-365, pSer376

Purpose: Doublecortin (phospho Ser339) Antibody

Specificity: Phospho-Doublecortin (S339) Polyclonal Antibody detects endogenous levels of Doublecortin protein only when phosphorylated at S339.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Doublecortin around the phosphorylation site of Ser376. AA range:330-365

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: Doublecortin

Background:

Synonyms: DCX; DBCN; LISX; Neuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X

Background: This gene encodes a member of the doublecortin family.The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain&quo

Gene Name: DCX

Protein Name: Neuronal migration protein doublecortin

Molecular Weight: 44 kDa

Gene ID: 1641

OMIM: 300067

UniProt: O43602