NCOA2 antibody (pSer736)

Catalog No. ABIN1532157

This Rabbit Polyclonal antibody specifically detects NCOA2 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NCOA2 antibody (pSer736) (ABIN1532157)

Target: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NCOA2 Antibody

Binding Specificity: AA 702-751, pSer736

Purpose: GRIP-1 (phospho Ser736) Antibody

Specificity: Phospho-GRIP-1 (S736) Polyclonal Antibody detects endogenous levels of GRIP-1 protein only when phosphorylated at S736.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human NCoA2 around the phosphorylation site of Ser736. AA range:702-751

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NCOA2

Target: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Alternative Name: NCoA2

Background:

Synonyms: NCOA2, BHLHE75, TIF2, Nuclear receptor coactivator 2, NCoA-2, Class E basic helix-loop-helix protein 75, bHLHe75, Transcriptional intermediary factor 2, hTIF2

Background: The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Gene Name: NCOA2

Protein Name: Nuclear receptor coactivator 2

Molecular Weight: 159 kDa

Gene ID: 10499

OMIM: 601993

UniProt: Q15596

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling