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Phospholipase D2 antibody (pTyr169)

This Rabbit Polyclonal antibody specifically detects Phospholipase D2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Rat and Mouse.
Catalog No. ABIN1532165

Quick Overview for Phospholipase D2 antibody (pTyr169) (ABIN1532165)

Target

See all Phospholipase D2 (PLD2) Antibodies
Phospholipase D2 (PLD2)

Reactivity

  • 48
  • 24
  • 22
  • 22
  • 18
  • 17
  • 17
  • 15
  • 2
  • 2
  • 1
Human, Rat, Mouse

Host

  • 41
  • 7
Rabbit

Clonality

  • 42
  • 6
Polyclonal

Conjugate

  • 23
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Phospholipase D2 antibody is un-conjugated

Application

  • 19
  • 16
  • 13
  • 13
  • 7
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Binding Specificity

    • 15
    • 10
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 136-185, pTyr169

    Specificity

    PLD2 (Phospho-Tyr169) Antibody detects endogenous levels of PLD2 only when phosphorylated at Tyr169.

    PhosphorylationH:Y169 M:Y169 R:Y169

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

    Comment

    Unigene-Number: Hs.104519 (NCBI Gene Symbol: PLD2)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Phospholipase D2 (PLD2)

    Alternative Name

    PLD2

    Background

    Synonyms: choline phosphatase 2, phosphatidylcholine-hydrolyzing phospholipase D2, phospholipase D2, PLD 2, PLD1C

    NCBI Gene Symbol: PLD2

    Molecular Weight

    105 kDa

    Gene ID

    5338

    OMIM

    602384

    UniProt

    O14939

    Pathways

    RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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