TK (AA 1-50) antibody

Catalog No. ABIN483033

The Rabbit Polyclonal anti-TK antibody (ABIN483033) specifically detects TK in ELISA, WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TK (AA 1-50) antibody (ABIN483033)

Target: TK

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 1-50

Purpose: Thymidine Kinase Antibody

Specificity: Thymidine Kinase Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TK. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: TK

Background:

Synonyms: TK1, Thymidine kinase, cytosolic

Background: catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,

Gene Name: TK1

Protein Name: Thymidine kinase cytosolic

Molecular Weight: 25 kDa

Gene ID: 7083

OMIM: 188300

UniProt: P04183