TLK1 antibody (AA 730-779)

Catalog No. ABIN1532489

The Rabbit Polyclonal anti-TLK1 antibody (ABIN1532489) specifically detects TLK1 in ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for TLK1 antibody (AA 730-779) (ABIN1532489)

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLK1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-TLK1 Antibody

Binding Specificity: AA 730-779

Purpose: TLK1 Antibody

Specificity: TLK1 Polyclonal Antibody detects endogenous levels of TLK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TLK1. AA range:730-779

Isotype: IgG

Application Details

Application Notes: IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: TLK1

Background:

Synonyms: TLK1, KIAA0137, Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHK1.,function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.,tissue specificity:Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.,

Gene Name: TLK1

Protein Name: Serine/threonine-protein kinase tousled-like 1

Molecular Weight: 86 kDa

Gene ID: 9874

OMIM: 608438

UniProt: Q9UKI8