PKC antibody (Thr497)

Catalog No. ABIN1532523

This Rabbit Polyclonal antibody specifically detects PKC in WB, IF, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC antibody (Thr497) (ABIN1532523)

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-PKC Antibody

Binding Specificity: Thr497

Specificity: PKC-pan Antibody detects endogenous levels of total PKC protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.

Comment:

Unigene-Number: Hs.531704, Hs.708867, Hs.460355, Hs.155342, Hs.580351, Hs.631564, Hs.333907, Hs.498570, Hs.496255 (NCBI Gene Symbol: PRKCA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20 °C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC

Background: Molecular weight of target protein: 76 kDa
Synonyms: KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta PKC-epsilon, PKCEA, PRKCE, epsilon type, kinase PKC-epsilon, nPKC-epsilon

Molecular Weight: 76 kDa

Gene ID: 5578, 5579, 5580

OMIM: 176960

UniProt: Q04759, P17252, P05129, Q05655, P05771, P24723, Q05513, Q02156

Pathways: Positive Regulation of Response to DNA Damage Stimulus