SIK1 antibody (AA 148-197)

Catalog No. ABIN1533113

This Rabbit Polyclonal antibody specifically detects SIK1 in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for SIK1 antibody (AA 148-197) (ABIN1533113)

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIK1 Antibody

Binding Specificity: AA 148-197

Purpose: SIK1 Antibody

Specificity: SIK1 Polyclonal Antibody detects endogenous levels of SIK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SIK. AA range:148-197

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for SIK1

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Alternative Name: SIK

Background:

Synonyms: SIK1, SIK, SNF1LK, Serine/threonine-protein kinase SIK1, Salt-inducible kinase 1, SIK-1, Serine/threonine-protein kinase SNF1-like kinase 1, Serine/threonine-protein kinase SNF1LK

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-182 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ.,subunit:Binds to and is activated by YWHAZ when phosphorylated on Thr-182.,

Gene Name: SIK1

Protein Name: Serine/threonine-protein kinase SIK1

Molecular Weight: 84 kDa

Gene ID: 150094

OMIM: 605705

UniProt: P57059

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process