Factor VIII antibody (AA 2161-2210)

Catalog No. ABIN1533268

The Rabbit Polyclonal anti-Factor VIII antibody (ABIN1533268) specifically detects Factor VIII in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for Factor VIII antibody (AA 2161-2210) (ABIN1533268)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VIII antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Factor VIII Antibody

Binding Specificity: AA 2161-2210

Purpose: Factor VIII Antibody

Specificity: Factor VIII Polyclonal Antibody detects endogenous levels of Factor VIII protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Factor VIII. AA range:2161-2210

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Alternative Name: Factor VIII

Background:

Synonyms: F8, F8C, Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component

Background: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation, factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008],

Gene Name: F8

Protein Name: Coagulation factor VIII

Molecular Weight: 267 kDa

Gene ID: 2157

OMIM: 306700

UniProt: P00451