MMP13 antibody (AA 10-59)

Catalog No. ABIN1533345

The Rabbit Polyclonal anti-MMP13 antibody is suitable to detect MMP13 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for MMP13 antibody (AA 10-59) (ABIN1533345)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MMP13 Antibody

Binding Specificity: AA 10-59

Purpose: MMP-13 Antibody

Specificity: MMP-13 Polyclonal Antibody detects endogenous levels of MMP-13 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MMP-13. AA range:10-59

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13

Background:

Synonyms: MMP13, Collagenase 3, Matrix metalloproteinase-13, MMP-13

Background: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016],

Gene Name: MMP13

Protein Name: Collagenase 3

Molecular Weight: 53 kDa

Gene ID: 4322

OMIM: 600108

UniProt: P45452