TIMP2 antibody (AA 21-70)

Catalog No. ABIN1533420

This Rabbit Polyclonal antibody specifically detects TIMP2 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 2+ publications

Quick Overview for TIMP2 antibody (AA 21-70) (ABIN1533420)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TIMP2 Antibody

Binding Specificity: AA 21-70

Purpose: TIMP-2 Antibody

Specificity: TIMP-2 Polyclonal Antibody detects endogenous levels of TIMP-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TIMP2. AA range:21-70

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-TIMP2 antibody (ABIN1533420)

Nishihara, Remacle, Angert, Shubayev, Shiryaev, Liu, Dolkas, Chernov, Strongin, Shubayev: "Matrix metalloproteinase-14 both sheds cell surface neuronal glial antigen 2 (NG2) proteoglycan on macrophages and governs the response to peripheral nerve injury." in: The Journal of biological chemistry, Vol. 290, Issue 6, pp. 3693-707, (2015) (PubMed).

Hu, Cai, Hu, Patel, Bard, Jamison, Coling: "Metalloproteinases and their associated genes contribute to the functional integrity and noise-induced damage in the cochlear sensory epithelium." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 43, pp. 14927-41, (2012) (PubMed).

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background:

Synonyms: TIMP2, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2

Background: This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008],

Gene Name: TIMP2

Protein Name: Metalloproteinase inhibitor 2

Molecular Weight: 24 kDa

Gene ID: 7077

OMIM: 188825

UniProt: P16035

Pathways: cAMP Metabolic Process