NDC80 antibody (AA 351-400)

Catalog No. ABIN1533486

This Rabbit Polyclonal antibody specifically detects NDC80 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for NDC80 antibody (AA 351-400) (ABIN1533486)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NDC80 Antibody

Binding Specificity: AA 351-400

Purpose: Hec1 Antibody

Specificity: Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: KNTC2

Background:

Synonyms: NDC80, HEC, HEC1, KNTC2, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC

Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011],

Gene Name: NDC80

Protein Name: Kinetochore protein NDC80 homolog

Molecular Weight: 73 kDa

Gene ID: 10403

OMIM: 607272

UniProt: O14777

Pathways: Maintenance of Protein Location