MID1 antibody (AA 71-120)

Catalog No. ABIN1533497

The Rabbit Polyclonal anti-MID1 antibody (ABIN1533497) specifically detects MID1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MID1 antibody (AA 71-120) (ABIN1533497)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MID1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MID1 Antibody

Binding Specificity: AA 71-120

Purpose: Midline-1 Antibody

Specificity: Midline-1 Polyclonal Antibody detects endogenous levels of Midline-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TRI18. AA range:71-120

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Background:

Synonyms: MID1; FXY; RNF59; TRIM18; XPRF; Midline-1; Midin; Midline 1 RING finger protein; Putative transcription factor XPRF; RING finger protein 59; Tripartite motif-containing protein 18

Background: midline 1(MID1) Homo sapiens The protein encoded byThis gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, t

Gene Name: MID1

Protein Name: Midline-1

Molecular Weight: 75 kDa

Gene ID: 4281

OMIM: 300000

UniProt: O15344