Topoisomerase II alpha antibody (AA 1-50)

Catalog No. ABIN1533609

This Rabbit Polyclonal antibody specifically detects Topoisomerase II alpha in WB, IHC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for Topoisomerase II alpha antibody (AA 1-50) (ABIN1533609)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1-50

Specificity:

TOP2A Antibody detects endogenous levels of total TOP2A protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TOP2A.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.156346 (NCBI Gene Symbol: TOP2A)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Synonyms: alpha isozyme, DNA topoisomerase II, DNA topoisomerase II-alpha, P11388-1, TOP-2, TOP2, TOP2A, TP2A

NCBI Gene Symbol: TOP2A

Molecular Weight: 174 kDa

Gene ID: 7153

OMIM: 126430

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases