PSKH1 antibody (AA 261-310)

Catalog No. ABIN1533610

The Rabbit Polyclonal anti-PSKH1 antibody is suitable to detect PSKH1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PSKH1 antibody (AA 261-310) (ABIN1533610)

Target: PSKH1 (Protein Serine Kinase H1 (PSKH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSKH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PSKH1 Antibody

Binding Specificity: AA 261-310

Purpose: PSK-H1 Antibody

Specificity: PSK-H1 Polyclonal Antibody detects endogenous levels of PSK-H1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human KPSH1. AA range:261-310

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PSKH1

Target: PSKH1 (Protein Serine Kinase H1 (PSKH1))

Background:

Synonyms: PSKH1, Serine/threonine-protein kinase H1, Protein serine kinase H1, PSK-H1

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activity depends on Ca(2+) concentration.,function:May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.,PTM:Autophosphorylated on serine residues.,PTM:Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.,PTM:Palmitoylated.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.,subunit:Homodimer.,tissue specificity:Expressed in all tissues and cell lines tested with the highest level of abundance in testis.,

Gene Name: PSKH1

Protein Name: Serine/threonine-protein kinase H1

Molecular Weight: 48 kDa

Gene ID: 5681

OMIM: 177015

UniProt: P11801