HARS1/Jo-1 antibody (AA 460-509)

Catalog No. ABIN1533611

The Rabbit Polyclonal anti-HARS1/Jo-1 antibody (ABIN1533611) specifically detects HARS1/Jo-1 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for HARS1/Jo-1 antibody (AA 460-509) (ABIN1533611)

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HARS1/Jo-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HARS1/Jo-1 Antibody

Binding Specificity: AA 460-509

Purpose: HisRS Antibody

Specificity: HisRS Polyclonal Antibody detects endogenous levels of HisRS protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HARS1/Jo-1

Target: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Alternative Name: HARS

Background:

Synonyms: HARS, HRS, Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS

Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids.The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],

Gene Name: HARS

Protein Name: Histidine--tRNA ligase cytoplasmic

Molecular Weight: 57 kDa

Gene ID: 3035

OMIM: 142810

UniProt: P12081