NFYA antibody (AA 261-310)

Catalog No. ABIN1533666

This Rabbit Polyclonal antibody specifically detects NFYA in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFYA antibody (AA 261-310) (ABIN1533666)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-NFYA Antibody

Binding Specificity: AA 261-310

Purpose: NF-YA Antibody

Specificity: NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human NFYA. AA range:261-310

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA

Background:

Synonyms: NFYA, Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA

Background: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008],

Gene Name: NFYA

Protein Name: Nuclear transcription factor Y subunit alpha

Molecular Weight: 36 kDa

Gene ID: 4800

OMIM: 189903

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha