ACTN2/3 antibody (AA 31-80)

Catalog No. ABIN1533709

The Rabbit Polyclonal anti-ACTN2/3 antibody is suitable to detect ACTN2/3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for ACTN2/3 antibody (AA 31-80) (ABIN1533709)

Target: ACTN2/3 (Actinin, alpha 2/3 (ACTN2/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-ACTN2/3 Antibody

Binding Specificity: AA 31-80

Purpose: Actinin-α2/3 Antibody

Specificity: Actinin-α2/3 Polyclonal Antibody detects endogenous levels of Actinin-α2/3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Actinin alpha-2/3. AA range:31-80

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ACTN2/3

Target: ACTN2/3 (Actinin, alpha 2/3 (ACTN2/3))

Background:

Synonyms: ACTN2, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013],

Gene Name: ACTN2/ACTN3

Protein Name: Alpha-actinin-2/3

Molecular Weight: 103 kDa

Gene ID: 88, 89

OMIM: 102573

UniProt: P35609