AAK1 antibody (AA 271-320)

Catalog No. ABIN1533939

This Rabbit Polyclonal antibody specifically detects AAK1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for AAK1 antibody (AA 271-320) (ABIN1533939)

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-AAK1 Antibody

Binding Specificity: AA 271-320

Purpose: AAK1 Antibody

Specificity: AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for AAK1

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Alternative Name: AAK1

Background:

Synonyms: AAK1, KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1

Background: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors.This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],

Gene Name: AAK1

Protein Name: AP2-associated protein kinase 1

Molecular Weight: 93 kDa

Gene ID: 22848

UniProt: Q2M2I8