DOK3 antibody (AA 101-150)

Catalog No. ABIN1533971

The Rabbit Polyclonal anti-DOK3 antibody (ABIN1533971) specifically detects DOK3 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DOK3 antibody (AA 101-150) (ABIN1533971)

Target: DOK3 (Docking Protein 3 (DOK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DOK3 Antibody

Binding Specificity: AA 101-150

Purpose: Dok-3 Antibody

Specificity: Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DOK3. AA range:101-150

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DOK3

Target: DOK3 (Docking Protein 3 (DOK3))

Alternative Name: DOK3

Background:

Synonyms: DOK3, Docking protein 3, Downstream of tyrosine kinase 3

Background: domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function.,PTM:Constitutively tyrosine-phosphorylated.,PTM:On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP.,tissue specificity:Expressed in spleen.,

Gene Name: DOK3

Protein Name: Docking protein 3

Molecular Weight: 53 kDa

Gene ID: 79930

OMIM: 611435

UniProt: Q7L591