ATPBD3 antibody (AA 291-340)

Catalog No. ABIN1533976

The Rabbit Polyclonal anti-ATPBD3 antibody is suitable to detect ATPBD3 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for ATPBD3 antibody (AA 291-340) (ABIN1533976)

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATPBD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATPBD3 Antibody

Binding Specificity: AA 291-340

Purpose: ATPBD3 Antibody

Specificity: ATPBD3 Polyclonal Antibody detects endogenous levels of ATPBD3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ATPBD3. AA range:291-340

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ATPBD3

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Alternative Name: ATPBD3

Background:

Synonyms: CTU1, ATPBD3, NCS6, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1

Background: function:Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.,pathway:tRNA modification, 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.,similarity:Belongs to the ttcA family. CTU1/NCS6/ATPBD3 subfamily.,subunit:Component of a complex at least composed of URM1, CTU2/NCS2 and ATPBD3/NCS6. May for a heterodimer with CTU2/NCS2.,

Gene Name: CTU1

Protein Name: Cytoplasmic tRNA 2-thiolation protein 1

Molecular Weight: 36 kDa

Gene ID: 90353

OMIM: 612694

UniProt: Q7Z7A3