DOK4 antibody (AA 11-60)

Catalog No. ABIN1534026

This Rabbit Polyclonal antibody specifically detects DOK4 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for DOK4 antibody (AA 11-60) (ABIN1534026)

Target: DOK4 (Docking Protein 4 (DOK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DOK4 Antibody

Binding Specificity: AA 11-60

Purpose: Dok-4 Antibody

Specificity: Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DOK4. AA range:11-60

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DOK4

Target: DOK4 (Docking Protein 4 (DOK4))

Alternative Name: DOK4

Background:

Synonyms: DOK4, Docking protein 4, Downstream of tyrosine kinase 4, Insulin receptor substrate 5, IRS-5, IRS5

Background: domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.,tissue specificity:Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.,

Gene Name: DOK4

Protein Name: Docking protein 4

Molecular Weight: 37 kDa

Gene ID: 55715

OMIM: 608333

UniProt: Q8TEW6